Method for improving SERS signal intensity of protein by introducing ionic liquid

The invention discloses a method for improving protein SERS (Surface Enhanced Raman Scattering) signal intensity by introducing ionic liquid. According to the method, a titanium dioxide nanotube array is immersed in a mixed solution containing to-be-detected protein and ionic liquid [P6, 6, 6, 14] [FuA], and the SERS active substrate is prepared. The ionic liquid [P6, 6, 6, 14] [FuA] is introduced into the SERS active substrate, the interaction between the protein and the substrate is enhanced, the ionic conductivity is improved, the overall polarizability of a detection system is increased, and the SERS performance of the protein is improved. The method disclosed by the invention has relatively high sensitivity and detectability, and has a wide application prospect in the field of trace detection of protein. 本发明公开了一种通过引入离子液体提高蛋白质SERS信号强度的方法。所述方法将二氧化钛纳米管阵列浸没于含有待检蛋白和离子液体[P6,6,6,14][FuA]的混合溶液中，制备SERS活性基底。本发明将离子液体[P6,6,6,14][FuA]引入SERS活性基底，增强蛋白与基底间的相互作用并提高了离子电导率，增加了检测体系整体的极化率，提高了蛋白的SERS性能。本发明方法具有较高的灵敏度和检测性，在蛋白的痕