Method for cultivating apoaequrin transgenic tomato and application thereof

The invention discloses a method for cultivating apoaequrin transgenic tomatoes and application of the method, and the method comprises the following steps: (1) constructing an apoaequrin transgenic tomato recombinant expression vector which comprises an apoaequrin coding gene (AQ), a 35S promoter sequence and a kanamycin resistance gene sequence; (2) transforming tomato Micro-Tom by using the recombinant expression vector to obtain an F0 generation; (3) carrying out selfing on the generation F0 to obtain a generation F1, preliminarily screening a resistant strain, and carrying out PCR (Polymerase Chain Reaction) screening on a positive strain by adopting an AQ fragment as a primer; and (4) carrying out selfing on the positive F1 plant to obtain an F2 generation transgenic tomato plant capable of expressing apoaequrin. The apoaequrin transgenic tomato plant plays an important role in the aspects of tomato growth and development, biological and abiotic stress living body calcium imaging research and the like,