Tyrosinase directional immobilization method and carrier preparation

The invention relates to a tyrosinase directional immobilization method and carrier preparation, and belongs to the technical field of tyrosinase. Agarose is added in the process of synthesizing magnetic nano-particles through a chemical precipitation method, core-shell structure magnetic nano-particles coated with agarose are synthesized through a one-step method, epoxy chloropropane and iminodiacetic acid are sequentially grafted on agarose shells of the nano-particles, copper ions are complexed, and the copper ion complexed core-shell structure magnetic nano-particles are prepared. The method comprises the following steps: firstly, preparing a nano-particle, then directionally immobilizing tyrosinase with a histidine tag by utilizing the affinity acting force of copper ions on the nano-particle and the histidine tag at the C terminal of the tyrosinase to prepare the immobilized tyrosinase, so that the stability and the specific enzyme activity of the tyrosinase are improved, and meanwhile, a catalyst and a