Design, preparation method and application of caffeic acid-biotin small molecule probe

The invention discloses a caffeic acid-biotin small-molecule probe as well as a preparation method and application thereof. The caffeic acid small-molecule probe structurally comprises three parts, namely caffeic acid, biotin and a connecting chain. The caffeic acid small-molecule probe designed by the invention is used for researching a polyphenol-protein action mechanism, the result shows that the caffeic acid small-molecule probe is effectively enriched to protein acting with caffeic acid, and the small-molecule probe provides basic and template molecules for researching other polyphenol-protein action mechanisms and also provides a good tool for exploring other pharmacological action mechanisms of caffeic acid. The method has important academic research value and application prospect. 本发明公开了一种咖啡酸-生物素小分子探针及其制备方法和应用，咖啡酸小分子探针结构式包括咖啡酸、生物素和连接链三部分组成。本发明设计的咖啡酸小分子探针用于研究多酚-蛋白作用机制，结果表明其有效富集到与咖啡酸作用的蛋白，该小分子探针为研究其他多酚与蛋白作用机制提供基础和模板分子，同时也为探索咖啡酸其他药理作用机制提供良好工具，具有重要的学术研究价值和应用前景。