Rapid detection method for carp spring emia virus by applying aptamer gold probe technology

The invention relates to the technical field of virus detection, in particular to a rapid detection method for a carp spring emia virus by applying an aptamer gold probe technology, which comprises the following steps: step 1, heating a chloroauric acid solution to boiling, adding a trisodium citrate solution, and cooling to room temperature to obtain GNPS; step 2, characterizing the gold nanoparticles by using a projection electron microscope; step 3, adding the GNPS solution into a sulfydryl modified aptamer, wherein the concentration of the finally obtained gold nano aptamer is 9.2 nM; and 4, taking the gold nano aptamer and sterile deionized water, uniformly mixing the gold nano aptamer and the sterile deionized water with a proper amount of carp spring blood disease virus liquid, adding sodium chloride solutions with different concentrations, observing color change, and setting negative control. The method has the beneficial effects that the SVCV aptamer is connected with the gold nanoparticles to constr