Sensor detection kit based on 96-well plate immobilized molecular motor and method for in-vitro detection of P24 antigen

The invention discloses a sensor detection kit based on a 96-well plate immobilized molecular motor and a method for P24 antigen in vitro detection.The kit is composed of a molecular motor sensor detection plate, and the detection plate is formed by immobilizing an F0F1-ATP enzyme molecular motor on a 96-well ELISA plate; meanwhile, a detection probe (P24 antibody) is combined to form a molecular motor biosensor for capturing a target. The detection method mainly comprises the following steps: (1) placing a sample in the detection plate, and capturing a target object by the sensor; (2) adding an ATP (adenosine triphosphate) synthesis solution containing ADP (adenosine triphosphate), and reacting And (3) adding an ATP detection reagent, quickly and uniformly mixing, and starting a chemical light emission detector to measure the luminous intensity. According to the present invention, the stability and the uniformity of the 96-well plate immobilized molecular motor, the mutual conversion characteristic of the ki