Immunofluorescent staining kit and method for tissue biopsy pathological section

The invention discloses a tissue biopsy pathological section immunofluorescence staining kit and method. The method comprises the following steps: carrying out characteristic staining on nucleolus by using fluorescently-labeled monoclonal antibodies such as nucleolus fibrin and the like, and staining the nucleolus of a cell into red; dyeing the cell nucleus into blue by using a DAPI fluorescent dye; and redyeing with eosin dye, dyeing the cytoplasm or cell surface into green, and dyeing the elastic fiber or collagen fiber into green or yellow green. Pathological sections dyed by the dyeing kit and the dyeing method are bright in color, and normal cells and pathological cells are obviously distinguished. And the trouble of making a detection result by an inspector is reduced, so that a basis is provided for structural evolution analysis and drug development of human cells. 本发明公开了一种组织活检病理切片免疫荧光染色试剂盒及方法。本发明使用荧光标记的核仁纤维蛋白等单克隆抗体对核仁进行特征性染色，将细胞的核仁染成红色；用DAPI荧光染料将细胞核染成蓝色；再用伊红染料复染，将细胞浆或细胞表面染成绿色，并将弹力纤维或胶原纤维染成绿色或黄绿色。由本发明染