Method for detecting acrylamide content based on up-conversion nano biosensing system
The invention belongs to the technical field of food safety detection, and particularly relates to a method for detecting the content of acrylamide based on an up-conversion and silver nano-cluster specific system. The preparation method comprises the following steps: firstly, preparing a core-shell...
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| creator | ZHANG YUNLIAN DING XIAODAN TAN CHUAN SENG OUYANG QIN RONG YAWEN |
| description | The invention belongs to the technical field of food safety detection, and particularly relates to a method for detecting the content of acrylamide based on an up-conversion and silver nano-cluster specific system. The preparation method comprises the following steps: firstly, preparing a core-shell structure up-conversion nano material, and modifying silicon dioxide and amino groups on the surface of the core-shell structure up-conversion nano material; the surface-modified up-conversion nano material is combined with DNA1, and then an aptamer and DNA2 are added for combination to obtain a specific detection system; adding acrylamide solutions with different concentrations, and then removing the fallen aptamer and DNA2 chain; adding silver nitrate and sodium borohydride again, and synthesizing a silver nano-cluster by taking the residual DNA2 as a template; finally, measuring fluorescence intensity signals in systems with different acrylamide concentrations as ordinates, and establishing a standard curve by |
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| subjects | INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES MEASURING NANOTECHNOLOGY PERFORMING OPERATIONS PHYSICS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES TESTING TRANSPORTING |
| title | Method for detecting acrylamide content based on up-conversion nano biosensing system |
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