Method for detecting acrylamide content based on up-conversion nano biosensing system

The invention belongs to the technical field of food safety detection, and particularly relates to a method for detecting the content of acrylamide based on an up-conversion and silver nano-cluster specific system. The preparation method comprises the following steps: firstly, preparing a core-shell structure up-conversion nano material, and modifying silicon dioxide and amino groups on the surface of the core-shell structure up-conversion nano material; the surface-modified up-conversion nano material is combined with DNA1, and then an aptamer and DNA2 are added for combination to obtain a specific detection system; adding acrylamide solutions with different concentrations, and then removing the fallen aptamer and DNA2 chain; adding silver nitrate and sodium borohydride again, and synthesizing a silver nano-cluster by taking the residual DNA2 as a template; finally, measuring fluorescence intensity signals in systems with different acrylamide concentrations as ordinates, and establishing a standard curve by