Triclocarban metabolic plasmid pDCA-TCC and application thereof

The invention discloses a triclocarban metabolic plasmid pDCA-TCC and an application of the triclocarban metabolic plasmid pDCA-TCC. The invention provides a plasmid pDCA-TCC for coding a triclocarban degradation pathway and a preparation method of the plasmid pDCA-TCC. The strain Pseudomonas putida...

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Bibliographische Detailangaben
Hauptverfasser: DONG HAORAN, QIAO WENJING, KE ZHUANG, CHEN KAI, ZHANG LENING, LUO ZIRUI, JIANG JIANDONG, ZHU WENQI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a triclocarban metabolic plasmid pDCA-TCC and an application of the triclocarban metabolic plasmid pDCA-TCC. The invention provides a plasmid pDCA-TCC for coding a triclocarban degradation pathway and a preparation method of the plasmid pDCA-TCC. The strain Pseudomonas putida KT2440 carrying a metabolic plasmid pDCA-TCC has a wide chloroaniline degradation substrate spectrum, not only can efficiently degrade an antibacterial agent triclocarban, but also can degrade toxic and harmful compounds such as a herbicide aniline and chlorpropham, a plant regulator forchlorfenuron, an insecticide diflubenzuron and the like. In urban domestic wastewater, the degrading bacterium KT2440 (pDCA-TCC) can be used for completely degrading 50 [mu] M of triclocarban within 3 days. The invention has good application potential and value in biologically enhanced remediation of triclocarban residue pollution, and has important significance in the aspects of protecting ecological environment safety, human heal