Strain for producing beta-carotene and application thereof

The invention relates to a strain for producing beta-carotene, which expresses a beta-carotene synthesis gene, overexpresses a mevalonic acid pathway related gene and knockout an acetyl coenzyme A metabolism related gene, so that a beta-carrot synthesis pathway and an endogenous mevalonic acid (MVA)...

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Bibliographische Detailangaben
Hauptverfasser:	WANG XUAN, WANG QINGXIA, LIU JIAHENG, DUAN XIAOGUO, PLUGOC CHENG, LIU YANFENG, SHI XUN, LI JIANGHUA, LYU XUEQIN, PEI TONGTONG, CHEN JIAN, LIU LONG, SUN XIAORUI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a strain for producing beta-carotene, which expresses a beta-carotene synthesis gene, overexpresses a mevalonic acid pathway related gene and knockout an acetyl coenzyme A metabolism related gene, so that a beta-carrot synthesis pathway and an endogenous mevalonic acid (MVA) pathway for strengthening the synthesis of saccharomyces cerevisiae vitamin A are constructed in the strain. And the yield of beta-carotene is improved. The strain disclosed by the invention realizes de novo synthesis of beta-carotene, and the beta-carotene is well expressed in shake flask fermentation. 本发明涉及一种生产β-胡萝卜素的菌株，表达β-胡萝卜素合成基因，过表达甲羟戊酸途径相关基因，以及敲除乙酰辅酶A代谢相关基因，从而在菌株中构建了β-胡萝卜合成途径，强化酿酒酵母维生素A合成的内源甲羟戊酸(MVA)途径，提高了β-胡萝卜素的产量。本发明的菌株实现了从头合成β-胡萝卜素，在摇瓶发酵中表现良好。