SADS-CoVS1 protein antigen as well as preparation method and application thereof

According to the present invention, the S1 functional region of the S protein (Spike Glycoprotein) of the porcine acute diarrhea syndrome coronavirus (SADS-CoV) is selected to construct and express the recombinant antigen, and the SDS-CoV is selected to construct the recombinant antigen, according to the recombinant antigen, the His tag is added at the N end of the protein and is used for purifying or detecting the protein; a polypeptide chain forming a trimer is added at the C end of the protein, so that the expressed recombinant antigen can form a trimer structure and an antigen configuration similar to natural; the sulfydryl or the polypeptide chain is beneficial to directionally coating the surface of the gold or the surface of the elisa plate by the recombinant antigen; the recombinant antigen can be used for preparing a detection reagent for detecting a novel coronavirus serological antibody and preparing a specific antibody of a new swine acute diarrhea syndrome coronavirus S protein. 本发明选取猪急性腹泻综合征冠状病毒