Chimeric terminal deoxynucleotide transferases for template-free enzymatic synthesis of polynucleotides

The present invention relates to compositions and methods for template-free enzymatic synthesis of polynucleotides using a terminal deoxynucleotidyl transferase that is a chimera derived from variants of different species. 本发明涉及使用末端脱氧核苷酸转移酶无模板酶学合成多核苷酸的组合物和方法，所述末端脱氧核苷酸转移酶是源自不同物种的变体的嵌合体。...

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Hauptverfasser:	SOSKIN MIKHAIL, MCHALE, DANIEL, CHAMPION ELISE, JAZRI, FATEN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to compositions and methods for template-free enzymatic synthesis of polynucleotides using a terminal deoxynucleotidyl transferase that is a chimera derived from variants of different species. 本发明涉及使用末端脱氧核苷酸转移酶无模板酶学合成多核苷酸的组合物和方法，所述末端脱氧核苷酸转移酶是源自不同物种的变体的嵌合体。