Phosphorylated tau protein detection kit

The invention relates to a kit for diagnosing Alzheimer's disease markers, in particular to a kit for detecting phosphorylated tau protein, and further relates to application of fluorescent dye and specific magnetic beads in preparation of the kit for detecting the phosphorylated tau protein. The kit is used for detecting the phosphorylated tau protein based on a single molecule detection technology and a double-antibody sandwich method, the invention further relates to a single molecule detection system for detecting the phosphorylated tau protein, high sensitivity can be achieved while rapid detection is achieved, the CV value is small, the cost of the detection system is low, and the concentration of the phosphorylated tau protein can be accurately measured. 本发明涉及诊断阿尔茨海默症标志物的试剂盒，尤其涉及检测磷酸化tau蛋白的试剂盒，另外还涉及荧光染料和特定的磁珠在制备检测磷酸化tau蛋白的试剂盒中的用途。所述试剂盒基于单分子检测技术及双抗夹心法来检测磷酸化tau蛋白，还涉及用于检测磷酸化tau蛋白的单分子检测系统，能够在快速检测的同时实现高的灵敏度，且CV值小，而且检测系统的成本低，能够准确测定磷酸化tau蛋白的浓度。