Extraction and purification method of phellinus igniarius active substance polysaccharide

The invention discloses a phellinus igniarius active substance polysaccharide extraction and purification method, which comprises the following steps: low-temperature freeze-dried powder crushing, enzymolysis after ultraviolet radiation, hot water-ultrasonic extraction, phellinus igniarius crude pol...

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Hauptverfasser: BI YUGEN, XU XIANGYANG, XU QIAN, DING HONGZHEN, JIA SHIJIE, LEE BYUNG-EUN, GUO YANFENG, LI PEIJU, TIAN GEILIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a phellinus igniarius active substance polysaccharide extraction and purification method, which comprises the following steps: low-temperature freeze-dried powder crushing, enzymolysis after ultraviolet radiation, hot water-ultrasonic extraction, phellinus igniarius crude polysaccharide extraction, degreasing, depigmentation, deproteinization treatment and dialysis, and finally, high-purity phellinus igniarius active substance polysaccharide is obtained. According to the method disclosed by the invention, the phellinus igniarius polysaccharide is extracted to the greatest extent through ultraviolet radiation treatment, enzymolysis and ultrasonic combined extraction, and finally, the high-purity phellinus igniarius polysaccharide is obtained through degreasing, depigmentation and deproteinization treatment. The method provided by the invention is high in extraction rate, short in extraction time and convenient to operate, and the obtained phellinus igniarius polysaccharide is high in pu