Agrobacterium tumefaciens-mediated sugarcane callus efficient genetic transformation method

The invention discloses an efficient genetic transformation method for agrobacterium-mediated sugarcane calluses, and belongs to the field of tissue culture. According to the invention, by enhancing the Agrobacterium tumefaciens Vir gene induction activation level and the bacterial liquid dip dyeing strength of sugarcane calluses, the occurrence frequency of transgenic events is greatly improved; an antioxidant is added into subsequent co-culture, screening and differential culture media to inhibit callus browning caused by agrobacterium-enhanced dip dyeing, so that the survival rate and the differentiation capacity of the dip-dyed calluses are obviously improved; a double-marker screening system with a green fluorescent protein marker EGFP gene and a herbicide glufosinate-ammonium resistance marker Bar gene at the same time is adopted, positive transgenic calluses are visually and nondestructively screened by using an EGFP marker, positive transgenic differentiated seedlings are screened by using Bar marker