Method for preparing antibacterial peptide feed additive through duplex mixed fermentation

The invention belongs to the field of bioengineering manufacturing, and discloses a method for preparing an antibacterial peptide feed additive through duplex mixed fermentation, which comprises the steps of bacillus licheniformis duplex pediococcus acidilactici seed liquid preparation, bacillus lic...

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Hauptverfasser:	ZHANG JIE, LI GAOQIANG, LIU YONGLI, NIU JUNKE, DUAN DANXIA, LIAN HAOSHUAI, LIU ZULIN, CHANG YIHAI, WANG WENLONG, YUAN LIQIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FODDER FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the field of bioengineering manufacturing, and discloses a method for preparing an antibacterial peptide feed additive through duplex mixed fermentation, which comprises the steps of bacillus licheniformis duplex pediococcus acidilactici seed liquid preparation, bacillus licheniformis duplex pediococcus acidilactici fermentation liquid preparation, high-pressure homogenate wall breaking, separation, ultrafiltration purification, spray drying and the like. The specific bacillus licheniformis (CGMCC No.24209) and pediococcus acidilactici are selected for duplex mixed fermentation, so that the antibacterial peptide crude product obtained after duplex mixed fermentation is high in antibacterial titer and has a very good antibacterial effect on wheat gibberella; the characteristic that aerobic bacteria and anaerobic bacteria are complementary in physiological characteristics in the fermentation process of bacillus licheniformis and pediococcus acidilactici is utilized, so that the activity