Dopamine type 2 receptor stable expression cell strain

The invention relates to a recombinant expression vector and an expression system for expressing a dopamine type 2 receptor, and a cell with the recombinant expression vector or the expression system. According to the dopamine type 2 receptor stable expression cell strain provided by the invention,...

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Bibliographische Detailangaben
Hauptverfasser: FAN ZHIRAN, LI YUHUA, LIU JIANFENG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to a recombinant expression vector and an expression system for expressing a dopamine type 2 receptor, and a cell with the recombinant expression vector or the expression system. According to the dopamine type 2 receptor stable expression cell strain provided by the invention, a target gene is stably knocked into a cell genome by utilizing a gene editing method, can be stably copied along with cell replication, cannot be lost during cell strain passage, and can be used for drug development and function detection taking a dopamine type 2 receptor as a target spot. 本发明涉及表达多巴胺2型受体的重组表达载体、表达系统,以及具有该重组表达载体或表达系统的细胞。本发明提供的多巴胺2型受体稳定表达细胞株利用基因编辑法将目的基因稳定敲入到细胞基因组中,可很稳定地随着细胞复制而复制,不会出现在细胞株传代而丢失的情况,可以用于以多巴胺2型受体为靶点的药物开发和功能检测。