Recombinant production method of microbial transglutaminase

The invention relates to a recombinant production method of microbial transglutaminase. The recombinant production method comprises the following steps: respectively constructing recombinant plasmids from a mature MTG coding gene and a leading peptide Pro coding gene, transforming the recombinant plasmids into the same host cell for co-expression, and primarily purifying to obtain the active microbial transglutaminase. The MTG leading peptide Pro and Trx protein are fused and then expressed into a'molecular chaperone 'Trx-Pro, MTG is assisted in correct folding when co-expressed with mature MTG protein, damage of MTG to recombinant expression host cells is inhibited, effective soluble expression and one-step simple purification of microbial transglutaminase are achieved, preliminarily purified protein is obtained after simple purification of the one-step method, and the Trx-Pro-Trx-Pro protein is obtained. The recombinant transglutaminase with high purity and high activity is obtained by further treatment wit