HIV (Human Immunodeficiency Virus) antibody/antigen diagnostic kit and preparation method thereof

The invention discloses an HIV (Human Immunodeficiency Virus) antibody/antigen diagnostic kit and a preparation method thereof, and relates to the technical field of HIV antibody/antigen diagnosis. The kit comprises a pre-coated enzyme-linked plate coated with an HIV antigen and a P24 monoclonal antibody, a rabbit anti-P24 polyclonal antibody labeled by biotin, an enzyme-labeled HIV antigen, a washing solution, a color developing solution A, a color developing solution B, a stop solution, a diluent and a negative control, wherein the negative control solution is normal human serum; an HIV antibody positive control serum; and P24 antigen positive control serum. The rabbit anti-P24 polyclonal antibody is labeled with enzyme, so that the signal amplification effect is achieved, the sensitivity of P24 antigen detection is greatly improved, the kit is used for detecting the P24 antigen and the HIV antibody in the blood of an HIV infected person, compared with a method which can only detect the HIV antibody in the