Anemia screening kit for CRISPR and CAS9 targeted capture of long fragment DNA and method thereof

The embodiment of the invention relates to the technical field of biological detection, in particular to an anemia screening kit for capturing long-fragment DNA in a targeted mode through CRISPR and CAS9. The anemia screening kit comprises sgRNA used for capturing thalassemia related genes in a targeted mode, a Y-type connector used for cyclization amplification and a primer used for cyclization amplification. The target capture range of the sgRNA is as follows: chr11: 5166361 to 5296578 (hg38), and chr16: 48994 to 210817 (hg38), and the target capture range of the sgRNA is as follows: chr11: 5166361 to 5296578 (hg38). According to the anemia screening kit for target capture of long fragment DNA by CRISPR and CAS9 and the method thereof, a large fragment region of thalassemia variation is captured in a targeted manner through CRISPR and CAS9 systems, five types of thalassemia mutation regions including alpha, beta, delta, delta beta and epsilon gamma delta beta are covered, and a three-generation or four-gene