Hybridoma cell strain 8D3, monoclonal antibody and application thereof

The invention provides a hybridoma cell strain 8D3, a monoclonal antibody and application of the monoclonal antibody, and belongs to the technical field of Ruan virus detection. The hybridoma cell strain 8D3 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.45105. The hybridoma cell strain 8D3 can efficiently and stably secrete the monoclonal antibody, the monoclonal antibody obtained through secretion is good in specificity, prion protein can be specifically recognized, and mad cattle disease and sheep itch disease pathogens can be distinguished. 本发明提供了一种杂交瘤细胞株8D3、单克隆抗体及其应用，属于阮病毒检测技术领域。本发明所述杂交瘤细胞株8D3保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCCNo.45105。本发明所述杂交瘤细胞株8D3能高效稳定的分泌单克隆抗体，分泌获得的单克隆抗体特异性好，不仅能特异性识别朊病毒蛋白，还能区分疯牛病和羊痒病病原体。