Functional specific PCR (Polymerase Chain Reaction) molecular marker of rice blast-resistant gene Pi25 and application of functional specific PCR molecular marker

The invention provides a functional specific PCR (Polymerase Chain Reaction) molecular marker of a rice blast-resistant gene Pi25 and application of the functional specific PCR molecular marker. The functional specific PCR molecular marker is characterized by a primer. The marker is used for carrying out PCR amplification and agarose electrophoresis on DNA extracted in the rice seedling stage, whether a to-be-detected material carries the rice blast-resistant allele Pi25 or not can be detected, the operation is simple, safety and economy are achieved, the accuracy is high, the specificity is high, and the marker can be applied to molecular marker-assisted selective breeding of the rice blast-resistant allele Pi25 and rice germplasm resource screening and identification. 本发明提供了水稻抗稻瘟病基因Pi25的功能特异性PCR分子标记及其应用，特征在于其引物。利用该标记对水稻苗期所提取的DNA进行PCR扩增和琼脂糖电泳，可以检测待测材料是否携带水稻抗稻瘟病等位基因Pi25，操作简单、安全经济、且准确性高、特异性强，可应用于水稻抗稻瘟病基因Pi25的分子标记辅助选择育种以及水稻种质资源筛选鉴定。