Whole genome hydroxymethylation conversion reagent and application thereof

The invention discloses a whole genome hydroxymethylation conversion reagent and application thereof, the reagent comprises an oxidizing agent and a reducing agent, the oxidizing agent comprises a metal oxide, and the reducing agent comprises 2-methylpyridine borane. The whole-genome hydroxymethylation conversion reagent can specifically recognize and convert 5hmC, only hydroxymethylation-modified cytosine is converted into T, methylation-modified cytosine is not converted, and the problems that in the prior art, a methylation sequencing library constructed based on bisulfite conversion is unbalanced in basic group, high in specificity and high in specificity are solved. And the kit has the advantages of simple components, low cost and high efficiency. 本发明公开了一种全基因组羟甲基化转化试剂及其应用，所述试剂包括氧化剂和还原剂，所述氧化剂包括金属氧化物，所述还原剂包括2-甲基吡啶硼烷。本发明方案的全基因组羟甲基化转化试剂，可特异性地识别并转换5hmC，只将羟甲基化修饰的胞嘧啶转换成T，而甲基化修饰的胞嘧啶不进行转换，解决了现有技术中基于重亚硫酸氢盐转化构建的甲基化测序文库所存在的碱基不平衡，测序数据使用率低的缺陷，组分简单，成本低，效率高。