New coronavirus S protein total antibody ELISA detection kit and preparation method thereof

The invention provides a novel coronavirus S protein total antibody ELISA (enzyme-linked immunosorbent assay) detection kit and a preparation method thereof. The novel coronavirus S protein total antibody ELISA detection kit comprises negative and positive control substances, 5 (6)-carboxyl fluorescein succinimide ester labeled novel coronavirus S protein, an HRP (horse radish peroxidase) labeled anti-5 (6)-carboxyl fluorescein succinimide antibody, a single-component TMB (tetramethylbenzidine) developing solution and a stop solution. The method needs to be matched with an optical microplate reader to judge a detection result, is suitable for scientific research or clinical laboratory detection, has the characteristics of high detection flux, high positive detection rate, high sensitivity, good stability, low cost and the like, and can be used for rapidly screening new coronavirus infected persons from large-scale crowds. 本发明提供新冠病毒S蛋白总抗体ELISA检测试剂盒及其制备方法，该新冠病毒S蛋白总抗体ELISA试剂盒包括阴阳性对照物质、5(6)-羧基荧光素琥珀酰亚胺酯标记的新冠病毒S蛋白、