Method for measuring content of rosmarinic acid in Xinhuang tablet

The invention provides a method for determining the content of rosmarinic acid in Xinhuang tablets. The method is carried out by adopting a high performance liquid chromatography, and the chromatographic conditions are as follows: a chromatographic column is Welch AQ-C18, 4.6 * 250 mm and 5 [mu] m,...

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Hauptverfasser: CHE LI, HUANG JIAYAN, ZHAO JUNJIE, LIAO GENJIE, WANG SHAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a method for determining the content of rosmarinic acid in Xinhuang tablets. The method is carried out by adopting a high performance liquid chromatography, and the chromatographic conditions are as follows: a chromatographic column is Welch AQ-C18, 4.6 * 250 mm and 5 [mu] m, a mobile phase is acetonitrile and a 0.1% phosphoric acid aqueous solution in a ratio of 19: 81, the flow velocity is 0.8 mL/min, the detection wavelength is 342 nm, and the column temperature is 30 DEG C. The determination method is reliable, simple, convenient, high in specificity and good in repeatability, stability and precision, can be used as a method for determining the content of the Xinhuang tablets, provides technical support for determination of the content of rosmarinic acid in the Xinhuang tablets, and is used for improving the product quality of the Xinhuang tablets. 本发明提供了新癀片中迷迭香酸含量的测定方法。所述方法采用高效液相色谱法进行,色谱条件如下:色谱柱:Welch AQ-C18,4.6×250mm,5μm,流动相:乙腈:0.1%磷酸水溶液=19:81,流速:0.8mL/min,检测波长:342nm,柱温:30℃。该测定方法可