Detection method of glycosylated exosome PD-L1
The invention discloses a detection method of a glycosylated exosome PD-L1. The detection method comprises the following steps: 1) separating the exosome; 2) connecting a first conjugate with a first DNA, wherein the first conjugate can be combined with the glycosyl of the exosome; 3) adding a conju...
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| creator | XU YUANFENG SONG YANLING YANG CHAOYONG YOU ZHENLONG ZHU LIN ZHANG CHI LIN HAOTING |
| description | The invention discloses a detection method of a glycosylated exosome PD-L1. The detection method comprises the following steps: 1) separating the exosome; 2) connecting a first conjugate with a first DNA, wherein the first conjugate can be combined with the glycosyl of the exosome; 3) adding a conjugate of the first conjugate and the first DNA; (4) adding a PD-L1 aptamer; 5) diluting the reaction solution; 6) adding the connecting arm DNA; 7) connecting the first DNA with the PD-L1 aptamer by the DNA ligase under the action of a connecting arm; and 8) amplifying the connecting part of the first DNA complementary with the connecting arm and the PD-L1 aptamer, and detecting an amplification signal. According to the method, the glycosylated exosome PD-L1 is specifically detected, glycoprotein does not need to be separated and released, the detection sensitivity is high, the detection limit is 1.09 pg/mL, and the method is suitable for various biological systems.
本发明公开了一种糖基化外泌体PD-L1的检测方法，包括如下步骤：1)分离外泌体；2)连接第一偶合物与 |
| format | Patent |
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本发明公开了一种糖基化外泌体PD-L1的检测方法，包括如下步骤：1)分离外泌体；2)连接第一偶合物与</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Detection method of glycosylated exosome PD-L1 |
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