Preparation and application of clonorchis sinensis protein Cs144

The invention discloses preparation and application of clonorchis sinensis protein Cs144, and relates to the technical field of biological detection, a nucleotide sequence of a coding region of the clonorchis sinensis gene Cs144 is as shown in SEQ ID NO: 1, and an amino acid sequence of the clonorch...

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Bibliographische Detailangaben
Hauptverfasser:	WANG ZHAOJUN, LI QI, QIAN MENBAO, KAN SHUO, YAO YANHUA
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses preparation and application of clonorchis sinensis protein Cs144, and relates to the technical field of biological detection, a nucleotide sequence of a coding region of the clonorchis sinensis gene Cs144 is as shown in SEQ ID NO: 1, and an amino acid sequence of the clonorchis sinensis protein Cs144 is as shown in SEQ ID NO: 2; the invention discloses a recombinant plasmid containing a coding region nucleotide sequence of a clonorchis sinensis Cs144 gene and a preparation method of the recombinant plasmid. The invention further discloses application of the clonorchis sinensis protein Cs144 in preparation of a clonorchis sinensis infection diagnosis antigen and application in preparation of a clonorchis sinensis infection ELISA (enzyme-linked immuno sorbent assay) detection kit. The invention provides a recombinant expression clonorchis sinensis protein Cs144 which is used as an antigen to be applied to ELISA (Enzyme-Linked Immuno Sorbent Assay) detection for diagnosing liver fluke dis