Modified gamma delta cells and uses thereof

The invention relates to modified gamma delta cells and uses thereof. The present invention provides compositions and methods for treating cancer or infectious diseases in humans. The invention encompasses the production and administration of gamma delta T cells expressing a chimeric antigen receptor (CAR) comprising an antigen binding domain, a hinge domain, a transmembrane domain, a co-stimulatory signaling domain comprising or not comprising a CD3 zeta signaling domain. Expression of a CAR sequence omitting the CD3 zeta signaling domain in gamma delta T cells provides in vivo CAR-T therapy that will produce cytolysis only for target cells that provide ligands in order to activate the gamma delta T cell receptor (TCR). 本申请涉及修饰的γδ细胞及其用途。本发明提供了用于治疗人类中的癌症或感染性疾病的组合物和方法。本发明包括γδT细胞的产生和施用，所述γδT细胞表达嵌合抗原受体(CAR)，所述嵌合抗原受体(CAR)包含抗原结合结构域、铰链结构域、跨膜结构域、包含或不包含CD3ζ信号传导结构域的共刺激信号传导结构域。省略CD3ζ信号传导结构域的CAR序列在γδT细胞中的表达提供了体内CAR-T治疗，其将仅对提供配体以便活化γδT细胞受体(TCR)的靶细胞产生细胞溶解。