Method for establishing hevea brasiliensis embryogenic cell pure line with high somatic embryogenic capacity and maintaining embryogenic form of hevea brasiliensis embryogenic cell pure line

The invention provides a method for establishing a rubber tree embryogenic cell pure line with high somatic embryogenic capacity and maintaining the embryogenic form of the rubber tree embryogenic cell pure line, and relates to the technical field of plant tissue culture and cell engineering. The method specifically comprises the following steps: inducing a primary callus, obtaining an embryogenic callus, and carrying out low-density suspension culture on the embryogenic callus, so as to establish the embryogenic cell pure line with high somatic embryogenesis capability. The invention also provides a subculture preservation method and a subculture medium for maintaining the embryogenic cell pure line. By utilizing the method provided by the invention, the obtaining time of the fragile embryogenic callus of the rubber tree can be shortened to be within 6 months, and an embryogenic cell pure line with the somatic embryo induction rate as high as 100% is obtained; when the subculture medium provided by the inven