Sulfamethoxazole fluorescent probe as well as preparation method and application thereof

The invention discloses a sulfamethoxazole fluorescent probe as well as a preparation method and application of the sulfamethoxazole fluorescent probe, and the preparation method comprises the following steps: (1) preparing a sulfamethoxazole (SMX) fluorescent probe stock solution; (2) preparing a sulfamethoxazole fluorescent probe working solution; (3) culturing a human colonic adenocarcinoma cell line Caco-2 and a human non-small cell lung cancer cell line A549; (4) treating Caco-2 and A549 cells by using a sulfamethoxazole fluorescent probe working solution; (5) fluorescence imaging; and (6) data analysis. According to the present invention, the fluorescence labeling means is adopted to accurately observe the distribution of the sulfamethoxazole in the biological cells, such that the theoretical support is provided for the evaluation of the influence of the sulfamethoxazole on the biological cells, and the method has characteristics of simple operation step, high accuracy, short experiment period, good flu