Magnetic detection method for interaction of biomolecules

The invention provides a magnetic detection method for biomolecular interaction, which comprises the following steps of: a, marking or capturing biomolecular interaction pairs in a biological sample through superparamagnetic particles, so that the biomolecular interaction pairs are combined on the surface of a functionalized diamond containing an ensemble NV color center sensor; b, performing magnetic imaging on the superparamagnetic particles on the surface of the diamond containing the ensemble NV color center sensor by using a wide-field magnetic imaging microscope; and c, establishing a mapping relation between the obtained magnetic image and the distribution of the superparamagnetic particles through a deep learning model, carrying out inverse solution and reconstruction on the distribution of the superparamagnetic particles, then counting the number of the superparamagnetic particles on the surface of the diamond, and analyzing the interaction of the target biomolecules through quantitative data of the