Polyphosphate kinase 1 mutant and construction and application of producing strain thereof

The invention discloses a polyphosphate kinase 1 mutant and construction and application of a producing strain thereof, and belongs to the technical field of gene engineering. According to the invention, Escherichia coli polyphosphate kinase 1 is subjected to mutation modification treatment, L at the 246th site of wild polyphosphate kinase 1 is mutated into P, M at the 247th site of wild polyphosphate kinase 1 is mutated into V, a polyphosphate kinase 1 mutant is obtained, and the amino acid sequence of the polyphosphate kinase 1 mutant is shown as SEQ ID NO.3. Compared with wild type polyphosphate kinase 1, the polyphosphate kinase 1 mutant disclosed by the invention has the advantages that the enzyme activity, the pH adaptability and the hydrophilicity are remarkably improved. Aiming at the polyphosphate kinase 1 mutant, the invention also constructs a production strain of the polyphosphate kinase 1 mutant. The producing strain provided by the invention can specifically and exogenously express the high-acti