Method for producing polyphosphate kinase 1 mutant through fermentation
The invention discloses a method for producing a polyphosphate kinase 1 mutant by fermentation, which comprises the following steps: (1) inoculating a seed solution of polyphosphate kinase 1 mutant producing bacteria into a fermentation culture medium for fermentation culture, cooling to 21-23 DEG C...
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| creator | CAO HUAJIE GUO YONGSHENG XIE PEI YUE MINGRUI |
| description | The invention discloses a method for producing a polyphosphate kinase 1 mutant by fermentation, which comprises the following steps: (1) inoculating a seed solution of polyphosphate kinase 1 mutant producing bacteria into a fermentation culture medium for fermentation culture, cooling to 21-23 DEG C when the OD600 value is 0.40-0.60 after the fermentation solution is diluted by 100 times, adding IPTG (isopropyl-beta-d-thiogalactoside) into the system for induced culture, carrying out induction culture for 20-24 hours; the residual sugar content of the system is monitored in the culture process, when the residual sugar content of the system is smaller than or equal to 1.0 g/L, supplementation is started, and the residual sugar concentration in the system is kept at 0.5-1.0 g/L through fed-batch supplementation; and (2) carrying out bacterium breaking treatment on the culture subjected to induction culture, separating and collecting supernatant, thereby obtaining the culture solution containing the polyphosphat |
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the residual sugar content of the system is monitored in the culture process, when the residual sugar content of the system is smaller than or equal to 1.0 g/L, supplementation is started, and the residual sugar concentration in the system is kept at 0.5-1.0 g/L through fed-batch supplementation; and (2) carrying out bacterium breaking treatment on the culture subjected to induction culture, separating and collecting supernatant, thereby obtaining the culture solution containing the polyphosphat</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220705&DB=EPODOC&CC=CN&NR=114703160A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220705&DB=EPODOC&CC=CN&NR=114703160A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CAO HUAJIE</creatorcontrib><creatorcontrib>GUO YONGSHENG</creatorcontrib><creatorcontrib>XIE PEI</creatorcontrib><creatorcontrib>YUE MINGRUI</creatorcontrib><title>Method for producing polyphosphate kinase 1 mutant through fermentation</title><description>The invention discloses a method for producing a polyphosphate kinase 1 mutant by fermentation, which comprises the following steps: (1) inoculating a seed solution of polyphosphate kinase 1 mutant producing bacteria into a fermentation culture medium for fermentation culture, cooling to 21-23 DEG C when the OD600 value is 0.40-0.60 after the fermentation solution is diluted by 100 times, adding IPTG (isopropyl-beta-d-thiogalactoside) into the system for induced culture, carrying out induction culture for 20-24 hours; 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the residual sugar content of the system is monitored in the culture process, when the residual sugar content of the system is smaller than or equal to 1.0 g/L, supplementation is started, and the residual sugar concentration in the system is kept at 0.5-1.0 g/L through fed-batch supplementation; and (2) carrying out bacterium breaking treatment on the culture subjected to induction culture, separating and collecting supernatant, thereby obtaining the culture solution containing the polyphosphat</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for producing polyphosphate kinase 1 mutant through fermentation |
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