Method for producing polyphosphate kinase 1 mutant through fermentation

The invention discloses a method for producing a polyphosphate kinase 1 mutant by fermentation, which comprises the following steps: (1) inoculating a seed solution of polyphosphate kinase 1 mutant producing bacteria into a fermentation culture medium for fermentation culture, cooling to 21-23 DEG C when the OD600 value is 0.40-0.60 after the fermentation solution is diluted by 100 times, adding IPTG (isopropyl-beta-d-thiogalactoside) into the system for induced culture, carrying out induction culture for 20-24 hours; the residual sugar content of the system is monitored in the culture process, when the residual sugar content of the system is smaller than or equal to 1.0 g/L, supplementation is started, and the residual sugar concentration in the system is kept at 0.5-1.0 g/L through fed-batch supplementation; and (2) carrying out bacterium breaking treatment on the culture subjected to induction culture, separating and collecting supernatant, thereby obtaining the culture solution containing the polyphosphat