Anti-estradiol monoclonal antibody and application thereof

The invention discloses an anti-estradiol monoclonal antibody and an application thereof. The anti-estradiol monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain variable region are respectively as shown in SEQ ID No. 3 to SEQ ID No. 5; the amino acid sequences of the CDR1, the CDR2 and the CDR3 of the light chain variable region are respectively as shown in SEQ ID No. 6 to SEQ ID No. 8. The invention provides a monoclonal antibody with relatively high affinity and detection sensitivity to estradiol, and a foundation is laid for research, development and popularization of indirect competitive ELISA kits and colloidal gold test strips. The anti-estradiol monoclonal antibody provided by the invention has the characteristics of strong specificity, high sensitivity and the like, and can be used as a raw material for enzyme-linked immunosorbent assay and colloidal gold immunoassay. 本发明公开了一种抗雌二醇单克隆抗体及其用途。一种抗