Multiplex analyte detection method

The invention provides a multiple analyte detection method capable of effectively improving detection sensitivity. The multiple analyte detection method comprises the following steps: providing micron particles; the micrometer particle is coupled with at least one first ligand and comprises a body and a plurality of first protrusions formed on the surface of the body. Then, mixing the micron particles with various to-be-analyzed substances to form a first compound; thereafter, the first complex is mixed with a plurality of second ligands bearing a plurality of labels such that the plurality of second ligands bind to the plurality of analytes in the first complex and form a second complex. And finally, detecting a plurality of markers of a plurality of second ligands in the second complex. 本发明提供一种可有效提高检测灵敏度的多重分析物的检测方法，包括以下步骤：提供微米粒子。微米粒子耦合有至少一种第一配体，且包括本体以及形成于本体的表面的多个第一突起物。接着，将微米粒子与多种待分析物混合，以形成第一复合物。尔后，将第一复合物与带有多种标记的多种第二配体混合，以使多种第二配体结合至第一复合物中的多种待分析物并形成第二复合物。最后，对第二复合物中的多种第二配体的多种标记进行检测。