Molecular detection method using cell lysis composition for nucleic acid extraction

The present invention relates to a molecular detection method using a cell lysis composition for nucleic acid extraction, and more particularly, to a molecular detection method using a cell lysis composition for nucleic acid extraction, according to the present invention, a polymerase chain reaction...

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Hauptverfasser: PARK SO HYUN, KIM SE RYUN, PARK CHANG-JOO, JIN ZHANDI, OH, JAE HOON
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The present invention relates to a molecular detection method using a cell lysis composition for nucleic acid extraction, and more particularly, to a molecular detection method using a cell lysis composition for nucleic acid extraction, according to the present invention, a polymerase chain reaction is performed on a mixture containing Tween 20, which is a component for nucleic acid extraction, and a PCR buffer necessary for performing the polymerase chain reaction, thereby minimizing the time required for molecular detection without requiring a separate nucleic acid extraction process. And the cost of molecular detection can be reduced by maximally reducing the use of special equipment and consumables in extraction. 本发明涉及使用核酸提取用细胞裂解组合物的分子检测方法,特别是如下的使用核酸提取用细胞裂解组合物的分子检测方法,其可以通过对同时含有作为核酸提取用组分的Tween 20和进行聚合酶链式反应所必需的PCR缓冲剂的混合物进行聚合酶链式反应而最大限度地减少分子检测所用的时间,而无需单独的核酸提取过程,并且可以通过最大限度地减少提取中专用设备和消耗品的使用而减少分子检测的成本。