Method for preparing 5-methylpyrazine-2-carboxylic acid by utilizing immobilized escherichia coli cells

The invention discloses a method for preparing 5-methylpyrazine-2-carboxylic acid by using immobilized Escherichia coli cells, which comprises the following steps of: 1, respectively fermenting and culturing recombinant Escherichia coli cells for expressing xylene monooxygenase, benzyl alcohol dehyd...

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Bibliographische Detailangaben
Hauptverfasser:	JIA GUANYA, WU YANJUN, WANG JING, WANG JUN, GAN HAISHENG, NIU WEINING, LI HENG, HE WEIJUAN, ZHU ZHILONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method for preparing 5-methylpyrazine-2-carboxylic acid by using immobilized Escherichia coli cells, which comprises the following steps of: 1, respectively fermenting and culturing recombinant Escherichia coli cells for expressing xylene monooxygenase, benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase by using Escherichia coli BL21 (DE3), and centrifuging to obtain thalli; 2, re-suspending the thallus, and uniformly mixing and stirring the thallus with polyethyleneimine and glutaraldehyde to prepare immobilized recombinant escherichia coli cells; and step 3, taking 2, 5-dimethylpyrazine as a substrate, taking the immobilized recombinant escherichia coli cell as a catalyst, and carrying out catalytic synthesis to obtain the 5-methylpyrazine-2-carboxylic acid. The invention provides a construction method of recombinant escherichia coli capable of realizing efficient soluble expression of xylene monooxygenase (XMO), benzyl alcohol dehydrogenase (BADH) and benzaldehyde dehydro