Hybridoma cell strain and application thereof

The invention relates to a hybridoma cell strain and application thereof, and belongs to the field of biomedicine. The hybridoma cell strain disclosed by the invention is t-PAIC2 # and is preserved in China Center for Type Culture Collection on April 18, 2020, the preservation number is CCTCC NO: C202066, and the preservation address is Wuhan University, Wuhan, China. The invention also provides a tPAI-C monoclonal antibody secreted by the hybridoma cell strain and an application of the tPAI-C monoclonal antibody. The hybridoma cell strain disclosed by the invention can secrete monoclonal antibodies with high affinity to different epitopes of tPAI-C protein; when being used for detecting tPAI-C, the monoclonal antibody disclosed by the invention is not easily interfered by tPA and PAI-1 monomers and is high in accuracy. 本发明涉及一种杂交瘤细胞株及其应用，属于生物医学领域。本发明的杂交瘤细胞株为t-PAIC2#，已于2020年4月18日保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：C202066，保藏地址为中国武汉，武汉大学。本发明还提供了由该杂交瘤细胞株分泌的tPAI-C单克隆抗体及tPAI-C单克隆抗体的应用。本发明杂交瘤细胞株可分泌对tPAI-C蛋白不同表位具有高亲和力的单克隆抗