Method for extracting, separating and purifying glabridin from glycyrrhiza glabra residues

The invention discloses a method for extracting glabridin from glycyrrhiza glabra residues and separating and purifying the glabridin, which comprises the following steps: by taking glycyrrhiza glabra residues as a raw material, extracting the glabridin by using a choline chloride/propionic acid eutectic solvent under the assistance of ultrasonic waves, extracting an extracting solution by using ethyl acetate, and carrying out gradient rotary evaporation on an extracting agent phase to obtain a glabridin crude extract; the glabridin crude extract is adsorbed, separated and purified by using macroporous resin, so that relatively high purity and recovery rate of the glabridin are achieved; and carrying out ethanol sedimentation and centrifugation on the extract phase to obtain xylooligosaccharide, carrying out rotary evaporation on the supernatant, adding deionized water for sedimentation and centrifugation to obtain lignin, further removing moisture from the supernatant through a reverse osmosis membrane, and