DNA walker analysis and detection method for ultrasensitive detection of APE1

The invention discloses a DNA walker analysis and detection method for ultrasensitive detection of APE1. The method comprises the following steps: annealing a double-foot DNA walker and a closed chain block by using a TAE/Mg < 2 + > buffer solution to form a double-chain DNA walker/block; the method comprises the following steps: annealing DNA (deoxyribonucleic acid)-H1, DNA-H2, DNA-H3 and DNA-H4 by using a TAE/Mg < 2 + > buffer solution to form a hairpin DNA-H1, a hairpin DNA-H2, a hairpin DNA-H3 and a hairpin DNA-H4; reducing sulfydryl-modified DNA-H1 by using tris (2-carboxyethyl) phosphine, then dropping the reduced sulfydryl-modified DNA-H1 to the surface of a gold electrode, incubating for 10 hours, and then treating for 0.5-1 hour by using 6-sulfydryl-1-hexanol; the double-stranded DNA walker/block, APE1 enzymes with different concentrations and hairpin DNA-H2 are mixed and dripped to the surface of the gold electrode for reaction; mixing the hairpin DNA-H3 and the hairpin DNA-H4, and dropwise adding t