Transformation method of escherichia coli

The invention discloses a transformation method of escherichia coli, and relates to a transformation method of a microbial strain. The invention solves the problems of long reaction time and tedious process in the existing escherichia coli transformation technology. The method comprises the following steps: lightly mixing plasmids with 30 [mu] l of Escherichia coli, placing the mixture on ice, carrying out heat shock in a water bath at 42 DEG C, immediately placing the mixture back to the ice, and standing for 1 min; and finally, inoculating into a solid LB plate culture dish containing antibiotics for culturing. The method is short in reaction time, simple in process and beneficial to popularization and application. 一种大肠杆菌的转化方法，本发明涉及一种微生物菌种的转化方法。本发明解决了现有大肠杆菌转化技术存在的反应时间较长、过程繁琐的问题。本发明方法：首先将质粒与30ul大肠杆菌轻轻混合后放置冰上，然后在42℃水浴中进行热休克，然后马上放回冰中，静置1min；最后接种到含抗生素的固体LB平板培养皿中进行培养。本发明的方法反应时间短、过程简单，利于推广使用。