Method for preparing 4-O-methyl hematoxylin through separation

The invention discloses a method for separating and preparing 4-O-methyl hematoxylin, which combines a column chromatography technology and a preparative high performance liquid chromatography technology, and can separate and obtain 4-O-methyl hematoxylin with the purity of more than 95.0% from hematoxylon. The 4-O-methyl hematoxylin prepared through separation is high in purity, large in preparation amount, rapid, efficient and suitable for batch production of the 4-O-methyl hematoxylin. 本发明公开了一种分离制备4-O-甲基苏木素的方法，本发明将柱色谱技术和制备型高效液相色谱技术相结合，可以从苏木中分离得到纯度大于95.0％的4-O-甲基苏木素；本发明分离制备得到的4-O-甲基苏木素纯度高，且制备量大，快速高效，适合4-O-甲基苏木素的批量生产。