Primer probe composition and kit for detecting BRAF gene mutation as well as use method and application of primer probe composition and kit

The invention discloses a primer probe composition and a kit for detecting BRAF gene mutation as well as a use method and application of the primer probe composition and the kit, and belongs to the technical field of gene detection. The primer probe composition comprises a real-time fluorescent PCR (Polymerase Chain Reaction) primer and a detection probe for detecting a BRAF gene mutation site c.1919A > C, wherein the real-time fluorescent PCR primer comprises amino acid sequences as shown in SEQ ID No.1-2; and the detection probe comprises an amino acid sequence as shown in SEQ ID No.3. The kit can be used for rapidly and accurately detecting the c.1919Tgt on the NM001374258.1 transcript of the BRAF gene in a human whole blood sample in vitro; a pathogenic variation. The detection result can be used for auxiliary diagnosis of papillary thyroid carcinoma, colorectal cancer and melanoma. 本发明公开了一种检测BRAF基因突变的引物探针组合物、试剂盒及其使用方法和应用，属于基因检测技术领域。这种引物探针组合物包括检测BRAF基因突变位点c.1919A＞C的实时荧光PCR引物和检测探针，实时荧光PCR引物包括SEQ ID No.1～2所