Non-diagnostic purpose detection method of tetrodotoxin

The invention discloses a non-diagnostic detection method of tetrodotoxin. A sensitive and rapid competitive enzyme-linked immunosorbent assay is established by using obtained bacteriophages of mimic epitope peptides shown as SEQ ID NO: 1, 3, 5, 7, 9 or 11; the IC50 of the method is 9.8 ng/mL, and t...

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Bibliographische Detailangaben
Hauptverfasser:	WEI LIUNA, LEI HONGTAO, SHEN YUDONG, LI YARU, WANG HONG, LUO LIN, LI XIANGMEI, XU ZHENLIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a non-diagnostic detection method of tetrodotoxin. A sensitive and rapid competitive enzyme-linked immunosorbent assay is established by using obtained bacteriophages of mimic epitope peptides shown as SEQ ID NO: 1, 3, 5, 7, 9 or 11; the IC50 of the method is 9.8 ng/mL, and the detection range is 3.4 ng/mL to 52.8 ng/mL. Compared with a traditional immunoassay method based on hapten, the method is more sensitive; the method has good cross reaction on other marine toxins. 本发明公开了一种河豚毒素的非诊断目的的检测方法，其用得到的氨基酸序列如SEQID NO:1、3、5、7、9或11所示模拟表位肽的噬菌体建立了灵敏、快速的竞争型酶联免疫分析法；该方法IC50为9.8ng/mL，检测范围为3.4～52.8ng/mL。与传统的基于半抗原的免疫分析方法灵敏度相比，本方法更加灵敏；该方法对其他海洋毒素的交叉反应良好。