Method for extracting and purifying zinc-protoporphyrin IX in nitrate-free dry-cured ham

Method for extracting and purifying zinc-protoporphyrin IX in nitrate-free dry-cured ham

The invention discloses a method for extracting and purifying zinc-protoporphyrin IX in a nitrate-free dry-cured ham. The method comprises the following steps: removing fat from a biceps femoris muscle sample of the dry-cured ham, and performing vacuum freeze drying; the method comprises the followi...

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Hauptverfasser: YANG ZIJIANG, GU DAHAI, WANG GUIYING, GE CHANGRONG, PU YUEHONG, LIAO GUOZHOU
Format: Patent
Sprache:chi ; eng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SEPARATION
TESTING
TRANSPORTING
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creator YANG ZIJIANG
GU DAHAI
WANG GUIYING
GE CHANGRONG
PU YUEHONG
LIAO GUOZHOU
description The invention discloses a method for extracting and purifying zinc-protoporphyrin IX in a nitrate-free dry-cured ham. The method comprises the following steps: removing fat from a biceps femoris muscle sample of the dry-cured ham, and performing vacuum freeze drying; the method comprises the following steps: taking a biceps femoris muscle sample after freeze drying, and carrying out crude extraction on zinc-protoporphyrin IX in the sample by using a 90% acetone aqueous solution; diluting the crude extract with distilled water according to a ratio of 1: 1 (v/v), filtering with filter paper, and adding a sample to a reverse C18 solid-phase extraction small column for purification; and collecting the purified eluent, removing acetone in the purified eluent by using a nitrogen blowing concentrator, and transferring the purified eluent into a vacuum freeze dryer for freeze drying to obtain a zinc-protoporphyrin IX purified sample. The process for extracting the zinc-protoporphyrin IX in the nitrate-free dry-cured
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SEPARATION
TESTING
TRANSPORTING
title Method for extracting and purifying zinc-protoporphyrin IX in nitrate-free dry-cured ham
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