Construction method of human lung adenocarcinoma cell strain capable of stably expressing pemetrexed drug resistance

The invention relates to a construction method of a human lung adenocarcinoma cell strain capable of stably expressing pemetrexed drug resistance, and the construction method comprises the step of culturing the human lung adenocarcinoma cell strain by pemetrexed in a mode of changing the concentration, so as to obtain the human lung adenocarcinoma cell strain which can be stably passed and has the pemetrexed drug resistance. Compared with a traditional high-concentration repeated intermittent method and a low-concentration long-term stimulation method, the novel method for screening the drug-resistant cell strain is simple in steps and fixed in screening time. According to the method, a compliant-concentration drug screening method is adopted, and high-concentration stimulation is changed into low-concentration drug-resistant storage, so that a stable drug-resistant cell strain can be obtained. 本发明涉及稳定表达培美曲塞耐药性人肺腺癌细胞株的构建方法，构建方法包含培美曲塞以顺浓度变化的方式培养所述人肺腺癌细胞株，得到能够稳定传代的，且具有所述培美曲塞耐药性的所述人肺腺癌细胞株。本发明确立了一种新的筛选耐药细胞株的方法，对比