Difenoconazole hapten, difenoconazole artificial antigen, difenoconazole antibody, and preparation method and application of difenoconazole hapten and difenoconazole artificial antigen

The invention provides a difenoconazole hapten, an artificial antigen, an antibody and a preparation method and application of the difenoconazole hapten, the difenoconazole artificial antigen, the difenoconazole antibody and the preparation method and application of the difenoconazole hapten, the difenoconazole artificial antigen and the difenoconazole antibody are characterized in that two haptens, namely the hapten B-PH and the hapten B-SG are prepared, the hapten B-PH is coupled with carrier protein BSA to obtain the artificial antigen B-PH-BSA, and the specific antibody for detecting difenoconazole is further prepared. An artificial antigen B-SG-OVA is used as a coating antigen; the antibody has good sensitivity and specificity to difenoconazole, the semi-inhibitory concentration is 1.75 ng/mL, the lowest detection limit is 0.022 ng/mL, the quantitative detection range is 0.11-27.57 ng/mL, the antibody has no cross reaction to a difenoconazole analogue, and the difenoconazole can be specifically detected;