Cell nucleus extraction method applied to multiple varieties of citrus

The invention discloses a cell nucleus extraction method applied to multiple varieties of citrus. The method comprises the following steps: preparing a required lysis solution and a sterilization related container; the method comprises the following steps: quickly immersing and fixing tender citrus leaves with liquid nitrogen, quickly and fully grinding, and adding into a lysis solution for fully and uniformly mixing; using Miracloth to separate a cell nucleus from other non-nuclear materials; the centrifugal parameters of the centrifugal filtrate and the centrifugal tubes with different sizes are different, and in the whole centrifuging process, the impurities such as chloroplast are gradually separated by sucking and beating through the treated wide-mouth gun head, and the cell nucleus and other non-nuclear materials are finely separated, so that the cell nucleus with required quality and concentration is finally obtained; the citrus cell nucleus extraction method provided by the invention is wide in applic